Lam P, Patapoff TW. Split Cakes, Still Delicious. PDA J Pharm Sci Technol 2019 Jan-Feb;73(1):16-29. Epub 2018 Aug 29.
Rajagopal K, Chang D, Nayak P, Izadi S, Patapoff TW, Zhang J, Kelley R, Sreedhara A. Trehalose Limits Fragment Antibody Aggregation and Influences Charge Variant Formation in Spray-Dried Formulations at Elevated Temperatures. Molecular Pharmaceutics 2019 16 (1) 349-358.
Kinnunen-Bown H, Bonn C, Yohe S, Bumbaca-Yadav D, Patapoff TW, Daugherty A, Mrsny RJ. In vitro model for predicting bioavailability of subcutaneously injected monoclonal antibodies. J Control Release 2018 03 6;273:13-20. Epub 2018 Feb 6.
Koenig P, Lee CV, Walters BT, Janakiraman V, Stinson J, Patapoff TW, and Fuh G.
Mutational landscape of antibody variable domains reveals a switch modulating the interdomain conformational dynamics and antigen binding. PNAS, 2017 114 (4) E486-E495.
Hilario EC, Stern A, Wang CH, Vargas YW, Morgan CJ, Swartz TE, Patapoff TW. An Improved Method of Predicting Extinction Coefficients for the Determination of Protein Concentration. PDA J Pharm Sci Technol 2017 Mar-Apr;71(2):127-135. Epub 2016 Oct 27.
Yin J, Swartz TE, Zhang J, Patapoff TW, Chen B, Marhoul J, Shih N, Kabakoff B, Rahimi K.
Validation of a Spectral Method for Quantitative Measurement of Color in Protein Drug Solutions.
PDA J Pharm Sci Technol. 2016 Jul-Aug;70(4):382-91.
Swartz TE, Yin J, Patapoff TW, Horst T, Skieresz SM, Leggett G, Morgan CJ, Rahimi K, Marhoul J, Kabakoff B.
A Spectral Method for Color Quantitation of a Protein Drug Solution.
PDA J Pharm Sci Technol. 2016 Jul-Aug;70(4):361-81.
Lapelosa M, Patapoff TW, Zarraga IE.
Molecular simulations of micellar aggregation of polysorbate 20 ester fractions and their interaction with N-phenyl-1-naphthylamine dye.
Biophys Chem. 2016 Jun;213:17-24.
Gikanga B, Adeniji NS, Patapoff TW, Chih HW, Yi L.
Cathepsin B Cleavage of vcMMAE-Based Antibody-Drug Conjugate Is Not Drug Location or Monoclonal Antibody Carrier Specific.
Bioconjug Chem. 2016 Apr 20;27(4):1040-9.
Walters BT, Jensen PF, Larraillet V, Lin K, Patapoff T, Schlothauer T, Rand KD, Zhang J.
Conformational Destabilization of Immunoglobulin G Increases the Low pH Binding Affinity with the Neonatal Fc Receptor.
J Biol Chem. 2016 Jan 22;291(4):1817-25.
Connolly BD, Le L, Patapoff TW, Cromwell ME, Moore JM, Lam P.
Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.
J Pharm Sci. 2015 Dec;104(12):4170-84.
Lapelosa M, Patapoff TW, Zarraga IE.
Modeling of protein-anion exchange resin interaction for the human growth hormone charge variants.
Biophys Chem. 2015 Dec;207:1-6.
Kinnunen HM, Sharma V, Contreras-Rojas LR, Yu Y, Alleman C, Sreedhara A, Fischer S, Khawli L, Yohe ST, Bumbaca D, Patapoff TW, Daugherty AL, Mrsny RJ.
A novel in vitro method to model the fate of subcutaneously administered biopharmaceuticals and associated formulation components.
J Control Release. 2015 Sep 28;214:94-102.
Sharma VK, Patapoff TW, Kabakoff B, Pai S, Hilario E, Zhang B, Li C, Borisov O, Kelley RF, Chorny I, Zhou JZ, Dill KA, Swartz TE.
In silico selection of therapeutic antibodies for development: viscosity, clearance, and chemical stability.
Proc Natl Acad Sci USA. 2014 Dec 30;111(52):18601-6. Erratum in: Proc Natl Acad Sci USA. 2015 Dec 1;112(48):E6719.
Lapelosa M, Patapoff TW, Zarraga IE.
Molecular simulations of the pairwise interaction of monoclonal antibodies.
J Phys Chem B. 2014 Nov 20;118(46):13132-41
Zheng K, Yarmarkovich M, Bantog C, Bayer R, Patapoff TW
Influence of glycosylation pattern on the molecular properties of monoclonal antibodies
MAbs. 2014 May-Jun;6(3):649-58
Adem YT, Schwarz KA, Duenas E, Patapoff TW, Galush WJ, Esue O
Auristatin antibody drug conjugate physical instability and the role of drug payload
Bioconjug Chem. 2014 Apr 16;25(4):656-64
Adem YT, Molina P, Liu H, Patapoff TW, Sreedhara A, Esue O.
Hexyl glucoside and hexyl maltoside inhibit light-induced oxidation of tryptophan
J Pharm Sci. 2014 Feb;103(2):409-16
Rajagopal K, Wood J, Tran B, Patapoff TW, Nivaggioli T.
Trehalose limits BSA aggregation in spray-dried formulations at high temperatures: implications in preparing polymer implants for long-term protein delivery
J Pharm Sci. 2013 Aug;102(8):2655-66
Abbas SA, Sharma VK, Patapoff TW, Kalonia DS.
Characterization of antibody-polyol interactions by static light scattering: implications for physical stability of protein formulations
Int J Pharm. 2013 May 20;448(2):382-9
Esue O, Xie AX, Kamerzell TJ, Patapoff TW.
Thermodynamic and structural characterization of an antibody gel
MAbs. 2013 Mar-Apr;5(2):323-34
Kortkhonjia E, Brandman R, Zhou JZ, Voelz VA, Chorny I, Kabakoff B, Patapoff TW, Dill KA, Swartz TE.
Probing antibody internal dynamics with fluorescence anisotropy and molecular dynamics simulations
MAbs. 2013 Mar-Apr;5(2):306-22
Chaudhri A, Zarraga IE, Yadav S, Patapoff TW, Shire SJ, Voth GA.
The role of amino acid sequence in the self-association of therapeutic monoclonal antibodies: insights from coarse-grained modeling
J Phys Chem B. 2013 Feb 7;117(5):1269-79
Zhang L, Patapoff T, Farnan D, Zhang B.
Improving pH gradient cation-exchange chromatography of monoclonal antibodies by controlling ionic strength
J Chromatogr A. 2013 Jan 11;1272:56-64
Abbas SA, Gaspar G, Sharma VK, Patapoff TW, Kalonia DS.
Application of second-derivative fluorescence spectroscopy to monitor subtle changes in a monoclonal antibody structure
J Pharm Sci. 2013 Jan;102(1):52-61
Chaudhri A, Zarraga IE, Kamerzell TJ, Brandt JP, Patapoff TW, Shire SJ, Voth GA.
Coarse-Grained Modeling of the Self-Association of Therapeutic Monoclonal Antibodies
J Phys Chem B. 2012 Jul 19;116(28):8045-57
Lam P, Patapoff TW.
An improved method for visualizing the morphology of lyophilized product cakes
PDA J Pharm Sci Technol. 65(4):425-30, 2011
Abbas SA, Sharma VK, Patapoff TW, Kalonia DS.
Opposite effects of polyols on antibody aggregation: thermal versus mechanical stresses
Pharm Res. 29(3):683-94, 2012
Abbas SA, Sharma VK, Patapoff TW, Kalonia DS.
Solubilities and transfer free energies of hydrophobic amino acids in polyol solutions: importance of the hydrophobicity of polyols
J Pharm Sci. 100(8):3096-104, 2011
Sundaramurthi P, Patapoff TW, Suryanarayanan R. Crystallization of trehalose in frozen solutions and its phase behavior during drying. Pharm Res. 27(11):2374-83, 2010.
Brandt JP, Patapoff TW, Aragon SR. Construction, MD simulation, and hydrodynamic validation of an all-atom model of a monoclonal IgG antibody. Biophys J. 99(3):905-13, 2010.
Esue O, Kanai S, Liu J, Patapoff TW, Shire SJ. Carboxylate-dependent gelation of a monoclonal antibody. Pharm Res. 26(11):2478-85, 2009.
Lazar KL, Patapoff TW, Sharma VK. Cold denaturation of monoclonal antibodies. MAbs. 2(1):42-52, 2010.
Patapoff TW, Esue O. Polysorbate 20 prevents the precipitation of a monoclonal antibody during shear. Pharm Dev Technol. 14(6):659-64, 2009.
Connolly B, Patapoff TW, Wang YJ, Moore JM, Kamerzell TJ. Vibrational spectroscopy and chemometrics to characterize and quantitate trehalose crystallization. Anal Biochem. 399(1):48-57, 2010
Kanai S, Liu J, Patapoff TW, Shire SJ. Reversible self-association of a concentrated monoclonal antibody solution mediated by Fab-Fab interaction that impacts solution viscosity. J Pharm Sci. 97(10):4219-27, 2008.
Weck S. Cheung S. Hiraoka-Sutow M. Patapoff T. Semba CP. Alteplase as a catheter locking solution: in vitro evaluation of biochemical stability and antimicrobial properties. Journal of Vascular & Interventional Radiology. 16(3):379-83, 2005.
Semba CP. Weck S. Razavi MK. Setum CM. Patapoff T. Characterization Of Alteplase (tPA) Following Delivery Through The Angiojet Rheolytic Catheter. Journal of Endovascular Therapy: Official Journal of the International Society of Endovascular Specialists. 12(1):123-8, 2005.
Lin TP. Hsu CC. Kabakoff BD. Patapoff TW. Application of frequency-modulated spectroscopy in vacuum seal integrity testing of lyophilized biological products. PDA Journal of Pharmaceutical Science & Technology. 58(2):106-15, 2004.
Semba, C.P.;Weck, S., Patapoff, T. Alteplase: Stability and Bioactivity Following Dilution in Normal Saline, J. Vasc. Interven. Radiol. 14:99-102, 2003.
Semba, C.P.;Weck, S.;Razavi, M.K.;Tuomi, L., Patapoff, T. Tenecteplase (TNK): Stability and Bioactivity of Thawed or Diluted Solutions Used in Peripheral Thromoblysis, J. Vasc. Interven. Radiol. 14(4):475-9, 2003.
Semba CP. Weck S. Razavi MK. Tuomi L. Patapoff T. Tenecteplase: stability and bioactivity of thawed or diluted solutions used in peripheral thrombolysis. Journal of Vascular & Interventional Radiology. 14(4):475-9, 2003.
Patapoff, T.W., Overcashier, D.E. The Importance of Freezing on Lyophilization Cycle Development, BioPharm 15:16-21,72, 2002.
Quan, C.P. Wu, S. Dasovich, N. Hsu, C. Patapoff, T. Canova-Davis, E. Susceptibility of rhDNase I to Glycation in the Dry-Powder State, Analytical Chemistry 71:4445-4454, 1999.
Semba CP. Weck S. Patapoff T. Alteplase: stability and bioactivity after dilution in normal saline solution. Journal of Vascular & Interventional Radiology. 14(1):99-102, 2003.
Cromwell MEM. Patapoff TW. Moore JM. Thermodynamics and Kinetics of Dimer Dissociation of antiVEGF. Biochemistry 38:13960-13967, 1999.
Patapoff TW. Tani T. Cromwell MEM., A Low Volume, Short Pathlength Dynamic Light Scattering Sample Cell for Highly Turbid Suspensions. Analytical Biochemistry 270:338-340, 1999.
Overcashier DE. Patapoff TW. Hsu C., Lyophilization of Protein Formulations in Vials: Investigation of the Relationship between Resistance to Vapor Flow during Primary Drying and Small-Scale Product Collapse. J. Pharm. Sci. 88:688-695, 1999.
Tani TA. Moore JM. Patapoff TW. Single step method for the accurate concentration determination of polysorbate 80. Journal of Chromatography A. 786:99-106, 1997.
Gonda I. Patapoff T. Inhalation delivery and formulation issues in gene therapy in the respiratory tract. Book: Inhalation Delivery of Therapeutic Peptides & Proteins. Editor: Adjei & Gupta. Marcel Dekker Publishing.
Lam XM. Patapoff TW. Nguyen TH. The effect of benzyl alcohol on recombinant human interferon-gamma. Pharmaceutical Research.14(6):725-9, 1997
Strohmeier GR. Lencer WI. Patapoff TW. Thompson LF. Carlson SL. Moe SJ. Carnes DK. Mrsny RJ. Madara JL. Surface expression, polarization, and functional significance of CD73 in human intestinal epithelia. Journal of Clinical Investigation. 99(11):2588-601, 1997
Osaka G. Carey K. Cuthbertson A. Godowski P. Patapoff, T. Ryan A. Gadek T. Mordenti J. Pharmacokinetics, tissue distribution, and expression efficiency of plasmid (33P)DNA following intravenous administration of DNA/cationic lipid complexes in mice: use of a novel radionuclide approach. Journal of Pharmaceutical Sciences. 85:612-618, 1996
Li S. Patapoff TW. Overcashier D. Hsu C. Nguyen TH. Borchardt RT. Effects of reducing sugars on the chemical stability of human relaxin in the lyophilized state. Journal of Pharmaceutical Sciences. 85(8):873-7, 1996
Li S. Patapoff TW. Nguyen TH. Borchardt RT. Inhibitory effect of sugars and polyols on the metal-catalyzed oxidation of human relaxin. Journal of Pharmaceutical Sciences. 85(8):868-72, 1996
Daugherty AL. Patapoff TW. Clark RC. Sinicropi DV. Mrsny RJ. Compaction assay: a rapid and simple in vitro method to assess the responsiveness of a biopolymer matrix to enzymatic modification. Biomaterials. 16(7):553-8, 1995
Mrsny RJ. Lazazzera BA. Daugherty AL. Schiller NL. Patapoff TW. Addition of a bacterial alginate lyase to purulent CF sputum in vitro can result in the disruption of alginate and modification of sputum viscoelasticity.Pulmonary Pharmacology. 7(6):357-66, 1994
Resnick MB. Colgan SP. Patapoff TW. Mrsny RJ. Awtrey CS. Delp-Archer C. Weller PF. Madara JL. Activated eosinophils evoke chloride secretion in model intestinal epithelia primarily via regulated release of 5'-AMP.Journal of Immunology. 151(10):5716-23, 1993
Patapoff TW. Mrsny RJ. Lee WA. The application of size exclusion chromatography and computer simulation to study the thermodynamic and kinetic parameters for short-lived dissociable protein aggregates.Analytical Biochemistry. 212(1):71-8, 1993
Madara JL. Patapoff TW. Gillece-Castro B. Colgan SP. Parkos CA. Delp C. Mrsny RJ. 5'-adenosine monophosphate is the neutrophil-derived paracrine factor that elicits chloride secretion from T84 intestinal epithelial cell monolayers [see comments]. Journal of Clinical Investigation. 91(5):2320-5, 1993 May. Comment in: J Clin Invest 1993 May;91(5):1857-1858
Lee WA. Narog BA. Patapoff TW. Wang YJ. Intranasal bioavailability of insulin powder formulations: effect of permeation enhancer-to-protein ratio Journal of Pharmaceutical Sciences. 80(8):725-9, 1991
Patapoff TW. Thomas GA. Wang Y. Peticolas WL. Polarized Raman scattering from oriented single microcrystals of d(A5T5)2 and d(pTpT). Biopolymers. 27(3):493-507, 1988
Volwerk JJ. Mrsny RJ. Patapoff TW. Jost PC. Griffith OH. Multiple equilibria binding treatment of lipid and detergent interactions with membrane proteins. Application to cytochrome c oxidase solubilized in cholate. Biochemistry. 26(2):466-75, 1987
Kubasek WL. Wang Y. Thomas GA. Patapoff TW. Schoenwaelder KH. Van der Sande JH. Peticolas WL. Raman spectra of the model B-DNA oligomer d(CGCGAATTCGCG)2 and of the DNA in living salmon sperm show that both have very similar B-type conformations. Biochemistry. 25(23):7440-5, 1986
Patapoff TW. Turpin PY. Peticolas WL. The Use of a Kramers-Kronig Transform Technique to Calculate the Resonance Raman Excitation Profiles of Anharmonic Molecular Vibrations. Journal of Physical Chemistry. 90:2347-2351, 1986
Koenig T. Rudolf K. Chadwick R. Geiselmann H. Patapoff T. Klopfenstein CE. Generation and Characterization of 2,6 Azulylene. Journal of the American Chemical Society. 108:5024-5025, 1986.
Baldwin JE. Patapoff TW. Barden TC. Stereochemical Kinetics of the Thermal Stereomutations Interconverting Achiral Isomers of 1-Phenyl-1,2,3-trideuteriocyclopropane. Journal of the American Chemical Society. 106:1421-1426,1984.
Presentations / Invited Speaker
Biomolecular Interaction Technologies Center (BITC) Symposium (2013): "Elucidation of Antibody Motion Using All Atom Molecular Dynamics"
University of California – San Francisco, Department of Pharmaceutical Sciences, invited speaker for Advanced Drug Delivery Course offered every two years (2008, 2010, 2012 (delayed until 2013): "Advanced Drug Delivery – Monoclonal Antibodies"
Higher Order Structure of Protein Therapeutics Scientific Organizing Committee Member CASSS sponsored meeting (2011)
AAPS National Biotechnology Conference 2010: "Predicting Large-Scale Drug Substance Stability at Small-Scale"
WCBP/CASSS CMC Strategy Forum (2010): panel discussion member along with: Yves Aubin of Health Canada, Steven Berkowitz of Biogen Idec, Brigitte Brake of BfArM in Germany, Roman Drews of CBER, Chris Geddes of UMBI,, Emily Shacter of CDER, and Keith Webber of CDER.
University of Connecticut, Department of Pharmaceutical Sciences, invited speaker, 2010: "Dialysis vs. Diafiltration" and "Advanced Drug Delivery – Monoclonal Antibodies"
IBC-WCBP 2009: "Predicting Large-Scale Drug Substance Stability at Small-Scale"
University of Wisconsin, Department of Pharmaceutical Sciences, invited speaker, 2009: "Pharmaceutical Considerations of Monoclonal Antibodies"
Protein Particles and Immunogenicity of Therapeutic Proteins: Filling in the Gaps in Risk Evaluation and Mitigation (sponsored by CDER/FDA and IABS, 2009). “Case Studies Related to Particle Characterization”
WCBP/CASSS Biophysical Techniques Workshop co-leader 2009 with Daron Freedberg, CBER, FDA & Sarah Kennett, CDER, FDA.
Asilomar Process Sciences Offsite, 2007, "Stick This In Your Eye"
WCBP/CASSS Biophysical Techniques Workshop co-leader 2006 with Daron Freedberg, CBER, FDA
Asilomar Process Sciences Offsite, 2006, "Antibodies And Anions
Better Buffer Binding Behavior Charge Changing Challenges"
Society for Technology in Anesthesia Annual Meeting, 2006, "Illusions and Hallucinations", co-presented with Tom Bewley
I have no clue what happened between 2002 and 2005… I think that I was a Director at that time…
IBC's 7th International Antibody Production and Downstream Processing, 2001, "Understanding and Controlling Primary Drying During the Lyophilization of Antibodies".
IBC's 6th International Antibody Production and Downstream Processing, 2000, "The Stability and Physicochemical Characterization of rhuMAb-VEGF"
Raw Materials & Contract Services of Mammalian Cell Products, The Williamsburg BioProcessing Foundation, 1999, "Characterization of Bovine-Derived and Vegetable-Derived Polysorbate 80"
Western Regional AAPS, 1999, "Use of anti- VEGF in the Treatment of Cancer"
Genentech Process Science Offsite, 1997, "If You Can’t Stand The Heat Then Either Stay Out Of The Lyophilizer Or Be More Sublime"
Annual National Meeting of the American Chemical Society, 1996, "The Early Detection Of Aggregate Formation In Protein Formulations And Characterizing Highly Turbid Solutions Of DNA/Lipid Complexes"
Gene Therapy Conference sponsored by CHI, "Issues of NonViral Vectors"
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